Virus Testing and Therapy

The following testing and therapy services are available from the National Clean Plant Network for Fruit Trees (NCPN-FT),Washington State University, 24106 N. Bunn Road, Prosser, WA 99350. Further information on this program and related issues can be viewed on the Internet at: http://ncpn-ft.org/

Test protocols for virus and virus-like agents that affect tree fruit cultivars are:

  • Woody indicator tests in the greenhouse or field.
  • Herbaceous indicator tests in the greenhouse.
  • Polymerase chain reaction (PCR) tests for phytoplasma.
  • cRNA hybridization for selected viroids.

NCPN-FT also provides virus therapy for infected deciduous tree fruit cultivars of commercial importance.

Additional virus testing is provided by the WSU ELISA Testing Laboratory where a range virus assays are conducted using enzyme-linked immunosorbent assay (ELISA) or Polymerase Chain Reaction (PCR). Testing by this lab is available for the following viruses.

  • Apple trees: apple chlorotic leafspot virus, apple mosaic virus, tomato ringspot virus
  • Asparagus: asparagus virus 1, asparagus virus 2, tobacco streak virus
  • Beans: alfalfa mosaic virus, bean common mosaic virus, bean common necrosis virus, cucumber mosaic virus, peanut stunt virus, tobacco ringspot virus, tobacco streak virus, tomato ringspot virus
  • Corn: high plains disease virus
  • Grapes: grape leafroll virus
  • Stone fruit trees: apple mosaic virus, cherry leafroll virus, prune dwarf virus, Prunus necrotic ringspot virus, tomato ringspot virus
  • Peas: pea enation mosaic virus, pea leafroll virus, pea seedborne mosaic virus
  • Roses: apple mosaic virus, Prunus necrotic ringspot virus, tobacco streak virus
  • Small fruits and berries: tobacco streak virus, tomato ringspot virus
  • Vegetables: cucumber mosaic virus, squash mosaic virus, tobacco ringspot virus

Information on virus testing is available at:

http://cpcnw.wsu.edu/virus_lab/